Fig. 3. Contents of total protein, β-aminoisobutyric acid and total amino acids produced from glutamine metabolism in C₂C₁₂ myotubes cultivated in the absence of glutamine (no addition and no addition plus glutamine synthase inhibitor) for 48 hours. Fig.s refer to the following measurements: (A) total protein, (B) total amino acids produced from glutamine/glutamate metabolism (Sum of Glu, Asp, Asn, Ala, Pro, Orn, and Arg concentrations), and (C) β-aminoisobutyric acid. The experimental groups are: (0 mM) no glutamine addition in the medium and (0 mM with glutamine synthase inhibitor) no glutamine addition in the medium with the glutamine synthase inhibitor (L-methionine sulfoximine - MSO at 0.1 mM). Results are presented as mean ± standard error and were analyzed using Student's t-test (^#p<0.05).